Macrophage Differentiation Inducing Factor from Human Monocytic Cells Is Equivalent to Murine Leukemia Inhibitory Factor

作者：Tsutomu Abe, Masanobu Murakami, T. Sato, Masahiro Kajiki, M. Ohno, Ryoji Kodaira · 发表于：Journal of Biological Chemistry · 年份：1989 · DOI：10.1016/s0021-9258(18)81884-1 · 被引用次数：50 · 研究领域：Immune Cell Function and Interaction、Cytokine Signaling Pathways and Interactions、Acute Lymphoblastic Leukemia research

Human macrophage differentiation inducing factor (DIF) can induce differentiation of human myeloid leukemic cells into macrophage-like cells in vitro. A procedure is described for purification of DIF from serum-free human monocytic leukemia THP-1 cell-conditioned medium. The procedure included concentration of a conditioned medium by ultrafiltration, lentil lectin-Sepharose affinity chromatography, and fast protein liquid chromatography using Mono S and Mono Q. DIF-A of pI 9.0 and DIF-B of pI 8.8 were obtained after a final purification with a Mono Q column, and both DIF gave a single peak with a molecular weight of approximately 51,000 determined by gel chromatography. NH2-terminal amino acid analysis of DIF-A showed a noticeable homology with murine leukemia inhibitory factor. Human DIF-A was found to induce maturation of human and murine leukemic cells into both macrophage-like cells with nitro blue tetrazolium reducing activity and phagocytic cells, but was found to suppress proliferation of these leukemic cells.