An Exonuclease Induced by Bacteriophage λ

作者：John William Little · 发表于：Journal of Biological Chemistry · 年份：1967 · DOI：10.1016/s0021-9258(18)96258-7 · 被引用次数：350 · 研究领域：Bacteriophages and microbial interactions、DNA and Nucleic Acid Chemistry、Advanced biosensing and bioanalysis techniques

λ exonuclease exhibits a much greater rate of attack on native deoxyribonucleic acid than on denatured DNA, the difference in rate being 350-fold for T7 phage DNA. The attack is exclusively exonucleolytic, and the only acid-soluble products are deoxyribonucleoside 5‘-monophosphates. In contrast to the known Escherichia coli exonucleases, its initial site of attack is at the 5‘ termini of a DNA molecule, with a strong preference for termini bearing a phosphoryl residue as compared with those bearing a free hydroxyl group.