Continuous-Flow Mechanical Cell Disintegrator

作者：John W. Ross · 发表于：Applied Microbiology · 年份：1963 · DOI：10.1128/am.11.1.33-35.1963 · 被引用次数：9 · 研究领域：Viral Infectious Diseases and Gene Expression in Insects、Microbial Inactivation Methods、Bacteriophages and microbial interactions

A mechanical cell disintegrator is described which efficiently ruptures bacteria, molds, actinomycetes, and yeasts. Cells were broken continuously in a chamber by rapid shaking of a mixture of cell suspension, plastic beads, and air. Volume capacity for complete breakage varied with experimental conditions and the microorganism involved, and ranged from 840 ml/hr to greater than 4,470 ml/hr. Subcellular preparations made with the machine retained a high level of enzymatic activity.