Phycocyanin synthesis and degradation in the blue-green bacterium Anacystis nidulans

作者：R. H. Lau, M M MacKenzie, W. Ford Doolittle · 发表于：Journal of Bacteriology · 年份：1977 · DOI：10.1128/jb.132.3.771-778.1977 · 被引用次数：89 · 研究领域：Photosynthetic Processes and Mechanisms、Biocrusts and Microbial Ecology、Protist diversity and phylogeny

Cellular content and rates of synthesis of the apoprotein subunits of phycocyanin in Anacystis nidulans cultures undergoing, and recovering from, nitrate starvation were measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of total and immunoprecipitable soluble proteins. Results indicated that (i) nitrate starvation provokes coordinate degradation of apoprotein subunits: (ii) de novo synthesis of these subunits is selectively depressed during starvation; (iii) nitrate restoration provokes coordinate increases in the rates of synthesis of these subunits, although maximal rates are not achieved for 6 to 10 h after readdition of nitrate; and (iv) illumination affects both relative and absolute rates of apoprotein formation.