Yeast mRNA splicing in vitro.

作者：Ren-Jang Lin, Andrew J. Newman, Soo-Chen Cheng, John N Abelson · 发表于：Journal of Biological Chemistry · 年份：1985 · DOI：10.1016/s0021-9258(17)38641-6 · 被引用次数：330 · 研究领域：RNA and protein synthesis mechanisms、RNA Research and Splicing、RNA modifications and cancer

Synthetic actin and CYH2 pre-mRNAs containing a single intron are accurately spliced in a soluble whole cell extract of yeast. Splicing in vitro requires ATP. The excised intron is released as a lariat in which an RNA branch connects the 5' end of the molecule to the last A in the "intron conserved sequence" UACUAAC. Two other discrete RNA species produced during splicing in vitro may represent reaction intermediates: free, linear exon 1 and a form of the intron lariat extending beyond the 3' splice site to include exon 2. Both lariat forms correspond to molecules previously shown to be produced during yeast pre-mRNA splicing in vivo.