Use of nuclepore filters for counting bacteria by fluorescence microscopy

作者：John E. Hobbie, Ralph J. Daley, S. Jasper · 发表于：Applied and Environmental Microbiology · 年份：1977 · DOI：10.1128/aem.33.5.1225-1228.1977 · 被引用次数：4282 · 研究领域：Microbial Community Ecology and Physiology、Traumatic Brain Injury and Neurovascular Disturbances、Cell Image Analysis Techniques

Polycarbonate Nuclepore filters are better than cellulose filters for the direct counting of bacteria because they have uniform pore size and a flat surface that retains all of the bacteria on top of the filter. Although cellulose filters also retain all of the bacteria, many are trapped inside the filter where they cannot be counted. Before use, the Nuclepore filters must be dyed with irgalan black to eliminate autofluorescence. Direct counts of bacteria in lake and ocean waters are twice as high with Nuclepore filters as with cellulose filters.