Rapid method for direct extraction of DNA from soil and sediments

作者：Y L Tsai, Betty H. Olson · 发表于：Applied and Environmental Microbiology · 年份：1991 · DOI：10.1128/aem.57.4.1070-1074.1991 · 被引用次数：622 · 研究领域：Microbial Community Ecology and Physiology、Bacteriophages and microbial interactions、Environmental DNA in Biodiversity Studies

A rapid method for the direct extraction of DNA from soil and sediments was developed. The indigenous microorganisms in the soil and sediments were lysed by using lysozyme and a freeze-thaw procedure. The lysate was extracted with sodium dodecyl sulfate and phenol-chloroform. In addition to a high recovery efficiency (greater than 90%), the yields of DNA were high (38 and 12 micrograms/g [wet weight] from sediments and soil, respectively). This method generated minimal shearing of the extracted DNA. The crude DNA could be further purified with an Elutip-d column if necessary. An additional advantage of this method is that only 1 g of sample is required, which allows for the analysis of small samples and the processing of many samples in a relatively short (7 h) period.