Use of pulsed-field gel electrophoresis for investigation of hospital outbreaks of Acinetobacter baumannii

作者：Anne Gouby, M J Carles-Nurit, Nicole Bouziges, Gisèle Bourg, Renaud Mesnard, Philippe J. M. Bouvet · 发表于：Journal of Clinical Microbiology · 年份：1992 · DOI：10.1128/jcm.30.6.1588-1591.1992 · 被引用次数：120 · 研究领域：Antibiotic Resistance in Bacteria、Bacterial biofilms and quorum sensing、Essential Oils and Antimicrobial Activity

Genomic DNAs from taxonomically and epidemiologically well-defined strains of Acinetobacter baumannii were digested with restriction endonucleases that cleave with low frequency, and the fragments were separated by pulse-field gel electrophoresis. Restriction fragment length polymorphisms were observed. Restriction fragment length polymorphism analysis can be used as an epidemiological tool to delineate outbreaks of nosocomial infections caused by A. baumannii.