Structure and organization of a mammalian 5 S gene cluster.

作者：Ronald P. Hart, William R. Folk · 发表于：Journal of Biological Chemistry · 年份：1982 · DOI：10.1016/s0021-9258(18)33820-1 · 被引用次数：60 · 研究领域：DNA Repair Mechanisms、Genomics and Chromatin Dynamics、Cancer-related Molecular Pathways

We have cloned and characterized a G+C-rich 2.2-kilobase pair Bam HI fragment which contains a single Syrian hamster 5 S rRNA gene. The cloned fragment directs the synthesis in vitro of 5 S RNA catalyzed by RNA polymerase III. Southern hybridization analysis of genomic hamster DNA indicates that the sequences present in the clone are tandemly repeated with a redundancy of 2700 copies per diploid cell.