Characteristics of a virus isolated from Hodgkin's disease lymph nodes.

作者：Warren L. Bostick, Lavelle Hanna · 发表于：PubMed · 年份：1955 · 被引用次数：9 · 研究领域：Lymphoma Diagnosis and Treatment、Virus-based gene therapy research、Herpesvirus Infections and Treatments

Summary Hodgkin9s disease and control human lymph node tissues have been studied and compared by intracerebral serial inoculations into suckling mice. After five to seven blind passages, a viral agent was detected in three cases of Hodgkin9s disease, two of which have been maintained and studied. Although the same mouse strain and technics were used in control material, no agent was encountered in it. The viruses isolated from the Hodgkin9s disease tissues produced a fatal encephalitis, manifest 7–10 days after inoculation. Only intracerebral injections were effective in the initially isolated strain. Intraperitoneal and intramuscular routes were not effective. After seventeen serial intracerebral passages in mice it became adapted to intraperitoneal transfer. Microscopically, the lesion was a diffuse severe encephalitis demonstrating, for the most part, a round-cell response. The spinal cord was also affected diffusely, so that the clinical symptoms were ataxia, flaccid paralysis, and death. Complete microscopic examination failed to reveal inflammation in muscles, fat, liver, pancreas, lungs, kidneys, etc. The agent was inactivated at 56° C. in 30 minutes and was resistant to ether. It produced no discernible lesions in chicken eggs or in tissue culture. Complement-fixing antibodies could be produced in immunized rabbits and, with difficulty, in young adult mice. No neutralizing antibodies have been produced in these animals. Human Hodgkin9s disease and control sera have no...