Amino Acid Analysis: Aqueous Dimethyl Sulfoxide As Solvent for the Ninhydrin Reaction

作者：Shannon J. Moore · 发表于：Journal of Biological Chemistry · 年份：1968 · DOI：10.1016/s0021-9258(18)94488-1 · 被引用次数：836 · 研究领域：Forensic Fingerprint Detection Methods

Abstract Methyl Cellosolve (the monomethyl ether of ethylene glycol) has been widely used as the organic solvent in ninhydrin reagents for amino acid analysis; it has, however, properties that are disadvantageous in a reagent for everyday employment. The solvent is toxic and it is difficult to keep the ether peroxide-free. A continuing effort to arrive at a chemically preferable and relatively nontoxic substitute for methyl Cellosolve has led to experiments with dimethyl sulfoxide, which proves to be a better solvent for the reduced form of ninhydrin (hydrindantin) than is methyl Cellosolve. Dimethyl sulfoxide can replace the latter, volume for volume, in a ninhydrin reagent mixture that gives equal performance and has improved stability. The result is a ninhydrin-hydrindantin solution in 75% dimethyl sulfoxide-25% 4 m lithium acetate buffer at pH 5.2. This type of mixture, with appropriate hydrindantin concentrations, is recommended to replace methyl Cellosolve-containing reagents in the quantitative determination of amino acids by automatic analyzers and by the manual ninhydrin method.