Hydroxyurea

作者：ROGER L. P. ADAMS, John Gordon LINDSAY · 发表于：Journal of Biological Chemistry · 年份：1967 · DOI：10.1016/s0021-9258(18)96181-8 · 被引用次数：198 · 研究领域：DNA and Nucleic Acid Chemistry、Metal complexes synthesis and properties、Synthesis and Biological Evaluation

Hydroxyurea inhibits deoxyribonucleic acid synthesis in mouse fibroblast (L) cells by inhibiting ribonucleotide reductase. No other site of action could be found. Inhibition can be reversed either by removal of the drug or by addition of deoxyribonucleosides. Although pyrimidine deoxyribonucleosides are readily phosphorylated by the cells, the corresponding purine compounds are largely converted to ribonucleotides. Following incubation for 18 hours in hydroxyurea, L cells washed free of inhibitor showed a burst of DNA synthesis lasting about 8 hours and involving 70% of the cells.