Isolation and characterization of a new pancreatic polypeptide hormone.

作者：JR Kimmel, L. J. Hayden, H. GAIL POLLOCK · 发表于：Journal of Biological Chemistry · 年份：1975 · DOI：10.1016/s0021-9258(19)40653-4 · 被引用次数：449 · 研究领域：Growth Hormone and Insulin-like Growth Factors、Neuropeptides and Animal Physiology、Pancreatic function and diabetes

A method is described for isolation, from chicken pancreas, of an avian pancreatic polypeptide which may be a new hormone. This method involves acid-alcohol extraction, gel filtration, DEAE-cellulose chromatography, and droplet countercurrent distribution. The peptide contains 36 amino acids, has a molecular weight of 4240 and the isoelectric point if pH 6 to 7. The average amount of avian pancreatic polypeptide extractable from chicken pancreas was 4 mg/100 g of pancreas. The amino acid sequence of the peptide is Gly-Pro-Ser-Gln-Pro-Thr-Tyr-Pro-Gly-Asp-Asp-Ala-Pro-Val-Glu-Asp-Leu-Ile-Arg-Phe-Tyr-Asp-Asn-Leu-Gln-Gln-Tyr-Leu-Asn-Val-Val-Thr-Arg-His-Arg-Tyr-NH2.