Estrogen Receptor in a Human Cell Line (MCF-7) from Breast Carcinoma

作者：Samuel Carroll Brooks, Elizabeth R. Locke, Herbert D. Soule · 发表于：Journal of Biological Chemistry · 年份：1973 · DOI：10.1016/s0021-9258(19)43537-0 · 被引用次数：415 · 研究领域：Plant-Derived Bioactive Compounds、Estrogen and related hormone effects、Cancer Cells and Metastasis

Abstract A stable cell line (MCF-7) derived by pleural effusion from a breast cancer patient has been demonstrated to contain significant amounts of 17β-estradiol receptor. This binding protein is specific for 17β-estradiol and has a Kd equal to 2.5 nm, a sedimentation constant of 4.0 S (and 9.2 S), and a mechanism capable of transporting the 17β-estradiol into the nucleus.