Aurovertin binds to the beta subunit of yeast mitochondrial ATPase

作者：Michael G. Douglas, Y. Koh, Michael E. Dockter, Gottfried Schatz · 发表于：Journal of Biological Chemistry · 年份：1977 · DOI：10.1016/s0021-9258(19)75220-x · 被引用次数：62 · 研究领域：Photosynthetic Processes and Mechanisms、Photoreceptor and optogenetics research、Mitochondrial Function and Pathology

Isolated beta subunit of ATPase (F1) from yeast mitochondria does not catalyze an ATPase reaction but still binds the specific F1 inhibitor aurovertin. Binding was measured by enhancement of aurovertin fluorescence; it was as tight as that to F1-ATPase. No binding was observed with F1 or with isolated beta subunit from a single-gene nuclear yeast mutant whose F1-ATPase was resistant to aurovertin.