Simple, rapid, and quantitative release of periplasmic proteins by chloroform

作者：Giovanna Ferro-Luzzi Ames, Catherine A. Prody, Sydney Govons Kustu · 发表于：Journal of Bacteriology · 年份：1984 · DOI：10.1128/jb.160.3.1181-1183.1984 · 被引用次数：350 · 研究领域：Porphyrin Metabolism and Disorders、Glycosylation and Glycoproteins Research、Hemoglobin structure and function

We introduce a method by which periplasmic proteins can be released rapidly, simply, and quantitatively by treating cells with chloroform. All the amino acid-binding proteins tested maintained their activity during chloroform treatment. This method makes practical the analysis of the periplasmic protein complement of a large number of strains.