A T4 Bacteriophage Mutant Which Lacks Deoxyribonucleic Acid Polymerase But Retains the Polymerase-associated Nuclease

作者：Nancy G. Nossal · 发表于：Journal of Biological Chemistry · 年份：1969 · DOI：10.1016/s0021-9258(19)78213-1 · 被引用次数：54 · 研究领域：Bacteriophages and microbial interactions、Monoclonal and Polyclonal Antibodies Research、Enzyme Structure and Function

Abstract Many T4 bacteriophage amber mutants in gene 43 do not induce the synthesis of either the phage DNA polymerase or the exonuclease which is physically inseparable from the polymerase. One mutant has been found in which a single amber mutation in gene 43 leads to the production of a protein which lacks the polymerase but retains the nuclease function of the wild type enzyme. The enzyme formed by this mutant in two unrelated nonpermissive hosts has chromatographic and enzymatic properties which differ somewhat from the wild type. Single step revertants of this mutant and infection of a permissive host by the mutant result in a polymerase and associated nuclease whose chromatographic and enzymatic properties are close to that of the wild type.