Rapid Mini-Prep Isolation of High-Quality Plasmid DNA from Lactococcus and Lactobacillus spp

作者：Daniel J. O’Sullivan, Todd R. Klaenhammer · 发表于：Applied and Environmental Microbiology · 年份：1993 · DOI：10.1128/aem.59.8.2730-2733.1993 · 被引用次数：382 · 研究领域：Probiotics and Fermented Foods、Genomics and Phylogenetic Studies、Biopolymer Synthesis and Applications

A simple, rapid plasmid mini-prep procedure for lactococci and lactobacilli which gives high yields and can be performed on overnight broth cultures is presented. Large plasmids were isolated from both lactococci and lactobacilli, including a 70-kb plasmid from Lactobacillus acidophilus C7. The purity of the resulting plasmid DNA makes it suitable for subsequent molecular manipulations. The convenience of the technique makes this rapid mini-prep procedure suitable for routine plasmid isolation from lactic acid bacteria.