The Fractionation of Arginine-rich Histones from Fetal Calf Thymus by Exclusion Chromatography

作者：Charles M. Mauritzen, Wesley C. Starbuck, Iengar S. Saroja, Charles W. Taylor, Harris Busch · 发表于：Journal of Biological Chemistry · 年份：1967 · DOI：10.1016/s0021-9258(18)96040-0 · 被引用次数：51 · 研究领域：Glycosylation and Glycoproteins Research、Genomics and Chromatin Dynamics、RNA and protein synthesis mechanisms

The arginine-rich histone complex from fetal calf thymus was found to contain 12 or more histone components by electrophoretic analysis on polyacrylamide gels. The arginine-rich histone complex was resolved into five distinct peaks b,y exclusion chromatography on Sephadex G-100 columns 300 cm in height and 7.6 cm in diameter, with 0.01 m HCl as the eluent. Of the five peaks obtained, two (Peaks 3 and 5) contained arginine-rich histone fractions. Peak 4 contained slightly lysine-rich histones. In Peaks 1 and 2, the arginine and lysine contents were almost equal. The last peak (Peak 5) contained a glycine-rich, arginine-rich histone in high purity as evidenced by electrophoretic homogeneity and peptide mapping.