Isolation and characterization of recombinant clones containing the chicken adult beta-globin gene.

作者：Gordon D. Ginder, William I. Wood, Gary Felsenfeld · 发表于：Journal of Biological Chemistry · 年份：1979 · DOI：10.1016/s0021-9258(19)86855-2 · 被引用次数：43 · 研究领域：Genomics and Chromatin Dynamics、Chromosomal and Genetic Variations、Plant Virus Research Studies

We have isolated and characterized two independent clones containing the chicken adult beta-globin gene. Each clone contains a 6.2-kilobase-pair Eco RI restriction fragment of chicken erythrocyte DNA inserted into the vector, lambda gtWES . lambda B. The orientation of the inserted fragment is opposite in the two clones. Characterization of the clones by electron microscopic R-loop studies, by restriction enzyme mapping, and by filter hybridization shows that the adult beta-globin gene is interrupted by at least one small and one large intervening sequence. In addition to the complete adult beta-globin gene, at least part of a second beta-globin-like gene was identified about 2.7 kilobase pairs from the 3'-end of the adult gene. The two independent clones, while very similar, do differ at two Msp I restriction endonuclease sites in regions flanking the adult beta-globin gene.