In vitro synthesis of an infectious RNA from cDNA clones of human rhinovirus type 14

作者：Satoshi Mizutani, Richard J. Colonno · 发表于：Journal of Virology · 年份：1985 · DOI：10.1128/jvi.56.2.628-632.1985 · 被引用次数：88 · 研究领域：Respiratory viral infections research、Viral Infections and Immunology Research、Animal Disease Management and Epidemiology

Development of a novel infectious cDNA assay is described for human rhinovirus type 14. A full-length cDNA clone of the human rhinovirus type 14 genome RNA was assembled and transcribed in vitro by using the SP6 transcription system. Transfection of HeLa cells with the nascent RNA resulted in the production of rhinovirus indistinguishable from the parental virus by both immunological and polyacrylamide gel analysis.