Characterization of two triacylglycerol lipase activities in pig post-heparin plasma

作者：Christian Ehnholm, André Bensadoun, W. Virgil Brown · 发表于：Biochemical Journal · 年份：1977 · DOI：10.1042/bj1630347 · 被引用次数：15 · 研究领域：Lipid metabolism and biosynthesis、Pharmacology and Obesity Treatment、Fatty Acid Research and Health

Two triacylglycerol lipase activities were characterized after partial purification from pig post-heparin plasma. These two lipase activities were eluted sequentially with a NaCl gradient from columns containing Sepharose with covalently linked heparin. The first lipase activity, which was eluted at 0.75M-NaCl, was not inhibited at 28 degrees C in the presence of 1M-NaCl and was not further activated by plasma apolipoproteins. The absence of this lipase activity from post-heparin plasma from hepatectomized pigs indicates that the liver plays a role in the synthesis of this enzyme. A second lipase activity, which was eluted at 1.2M-NaCl, was inhibited when assayed in the presence of 1.0M-NaCl and was activated 14-fold by an apolipoprotein isolated from human very-low-density lipoprotein. The characteristics are identical with those of lipoprotein lipase purified from pig adipose tissue.