The Ncm-1 gene for resistance to Cucumber mosaic virus in yellow lupin (Lupinus luteus): molecular studies and marker development

作者：Dora Li · 发表于：Murdoch Research Repository (Murdoch University) · 年份：2012 · 被引用次数：3 · 研究领域：Plant Virus Research Studies、Plant Disease Resistance and Genetics、Legume Nitrogen Fixing Symbiosis

Cucumber mosaic virus (CMV) is an important virus pathogen of lupins in Australia which causes serious yield losses of up to 60% in epidemic years. In commercially grown lupin (Lupinus angustifolius and L. luteus) crops CMV is spread non-persistently by aphid vectors, but it can also be seed borne and this extends virus infection into successive generations. Resistance to CMV has been identified in L. luteus cv. Wodjil and is the conferred by the Ncm-1 gene. The aims of this research were to study the Ncm-1 gene in order to gain a better understanding of resistance in yellow lupin, and to develop a molecular marker linked to Ncm-1 for use in marker assisted selection.
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\nPreviously published data by Jones et al (1996) identified Ncm-1 as being a single dominant resistance gene, however, phenotypic analysis of CMV infection in a segregating L. luteus mapping population in this thesis was consistent with the Ncm-1 gene being a dominant gene modified by at least one other minor gene. The polygenic nature of CMV resistance in this genetic background was further supported by AFLP analysis which identified one major and one minor QTL associated with resistance.
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\nA PCR based approach, using degenerate primers designed on conserved disease resistance protein motifs, was used to identify resistance gene analogues (RGA) in L. luteus. Comparative analysis revealed that RGAs isolated from L. luteus were members of the TIR-NBS-LRR class of R proteins and were similar...