Automated High-Throughput Sex-Typing Assay

作者：Lizhi Yu, Fernando D. Martínez, Walter T. Klimecki · 发表于：BioTechniques · 年份：2004 · DOI：10.2144/04374dd01 · 被引用次数：8 · 研究领域：Epigenetics and DNA Methylation、Sex work and related issues、Sex and Gender in Healthcare

BioTechniquesVol. 37, No. 4 Drug Discovery and Genomic TechnologiesOpen AccessAutomated high-throughput sex-typing assayLizhi Yu, Fernando D. Martinez & Walter T. KlimeckiLizhi YuArizona Respiratory Center, Tucson, Arizona, USA, Fernando D. MartinezArizona Respiratory Center, Tucson, Arizona, USA & Walter T. Klimecki*Address correspondence to: Walt Klimecki, Arizona Respiratory Center, P.O. Box 245030, Tucson, Arizona 85641, USA. e-mail: E-mail Address: walt@resp-sci.arizona.eduArizona Respiratory Center, Tucson, Arizona, USAPublished Online:6 Jun 2018https://doi.org/10.2144/04374DD01AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInRedditEmail Determining the sex of the donor of a human DNA sample in a rapid and accurate manner is of importance in several settings, including basic research, clinical practice, and forensics (1,2). Our group routinely performs high-throughput genotyping within large epidemiological studies. It is not uncommon to receive thousands of DNA samples, with the tube identification as the only accompanying information. These samples will then be distributed through a chain of multi-well plates, ultimately ending in 384-well PCR plates in which there is no individual identification for a given sample, only a plate identifier. In such cases, a basic test of incoming sample identification integrity and of sample processing/tracking integrity is to sex type both the original DNA sample tubes a...