Improved separation of sugars on the paper partition chromatogram

作者：M. A. Jermyn, Frederick A. Isherwood · 发表于：Biochemical Journal · 年份：1949 · DOI：10.1042/bj0440402 · 被引用次数：463 · 研究领域：Porphyrin Metabolism and Disorders、Biochemical Analysis and Sensing Techniques、RNA and protein synthesis mechanisms

SADANA I949 450-5 m,u.)since it yielded a singleband on mixed chromato- graphy with cryptoxanthin obtained from yellow maize (Sadana, 1946).Zone II.This was an orange-coloured band.The pigment was identified as neo-,-carotene U (maxima in light petroleum 480 and 450 mp.; Sadana, 1949).Zone III.This was reddish orange in colour.The pig- ment was extracted and identified as $-carotene by its absorption spectrum (maxima in light petroleum 482 and 452 mp) Zone IV.This was a brownish yellow band.The pigment was extracted and identified as neo-,B-carotene B (maxima in petroleum 470 and 443 m,u.).It yielded a single band on mixed chromatography with neo-,B-carotene B prepared by HCI treatment of ,-carotene (Sadana, 1949).Results of analyses of six varieties of loquats varying in colour from pale yellow to deep orange are shown in Table 1. SUMMARYPigments separated chromatographically andidenti- fled from different varieties of loquats include, besides 'xanthophylls other than cryptoxanthin', cryptoxanthin, a-carotene, neo-p-carotene U and neo-,B-carotene B. Among the pigments present, cryptoxanthin and fl-carotene are the principal ones forming 15-7-33*8 and 36-5-59-6 % of the total pigments.