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A Mild Procedure for the Isolation of Polydisperse Glycogen from Animal Tissues

作者:Ernest Bueding, Stanley A. Orrell · 发表于:Journal of Biological Chemistry · 年份:1964 · DOI:10.1016/s0021-9258(18)91125-7 · 被引用次数:93 · 研究领域:Plant-Derived Bioactive Compounds、Polyamine Metabolism and Applications、Biopolymer Synthesis and Applications

In an attempt to obtain a product more closely approaching the state of glycogen in intact animal tissues, a procedure for the extraction of this polysaccharide is described. This procedure is designed to avoid physical and chemical alteration by strong alkali, acid, or high temperature and distortion of the molecular weight spectrum resulting from selective losses during sedimentation. (Author)